Bladder cancer-associated transcript 2 contributes to nephroblastoma progression

Bladder Cancer

J Gene Med. 2020 Nov 3:e3292. doi: 10.1002/jgm.3292. Online ahead of print.


BACKGROUND: Nephroblastoma is a common pediatric kidney tumor. Existing evidence has indicated that long non-coding RNAs (lncRNAs) may be associated with tumorigenesis such as nephroblastoma. However, the contribution of lncRNA bladder cancer-associated transcript 2 (BLACAT2) to tumorigenesis and postoperative nephroblastoma prognosis remains unknown.

METHODS: A total of 50 pairs of patient nephroblastoma and corresponding adjacent non-tumorous tissues were analyzed for BLACAT2 expression. The underlying roles of BLACAT2 in nephroblastoma cells were also investigated. BLACAT2 level was detected in four nephroblastoma cell lines and normal cell line NGC-407 using quantitative real-time PCR. The potential influence of BLACAT2 on nephroblastoma cells was explored based on RNA interference technology in vitro and in vivo. Moreover, the miRNA targeted by BLACAT2 and its target gene were predicted and verified.

RESULTS: BLACAT2 silencing suppressed cell proliferation, colony formation, and tumor growth in vivo and promoted cell apoptosis in vitro. Furthermore, BLACAT2 could directly bind to miR-504-3p, thereby decreasing miR-504-3p expression. In addition, the impact of miR-504-3p on proliferation, colony formation, and nephroblastoma cell apoptosis was reversed by BLACAT2. Wnt11 was identified as a target of miR-504-3p.

CONCLUSIONS: Our study revealed that a novel BLACAT2/miR-504-3p/Wnt11 axis is associated with nephroblastoma, where BLACAT2 is able to sponge miR-504-3p to down-regulate Wnt11.