Transferrin receptors targeting peptide (T7 peptide) surface-modified sorafenib nanoliposomes enchance the anti-tumor effect in colorectal cancer

Colorectal Cancer
27/06/2020

Sun H and Wang S. Pharm Dev Technol 2020.

ABSTRACT

In this work, T7 modified nanoliposome loaded SNF (T7-SNF-NLPs) was developed. The physicochemical properties and characteristics of T7-SNF-NLPs, including morphology, particle size, zeta potential, stability, and in vitro release, were determined. In vitro toxicity and cellular uptake were evaluated in RKO cells. Antitumor efficiency was examined in RKO cells-bearing Kunming mice to assess their potential applications in the development of nanoliposomes therapeutics. The average particle size


of T7-SNF-NLPs was observed to be 131.6 ± 1.7 nm and the polydispersity index represented a uniform mono-dispersion with PDI =0.19. SNF was sustainably released from T7-SNF-NLPs at a release rate of 65% at 48 h in pH 7.4 PBS. The release rate of SNF was over 72% from T7-SNF-NLPs in pH 6.5 PBS, faster than that in pH 7.4, which indicated that the release rate of SNF was enhanced under the acidic environment. In vitro study clearly showed that T7 modified NLPs was more effective in inducing uptake and apoptosis in cancer cells than nonmodified NLPs. The IC50 values of T7-SNF-NLPs treated RKO cells was 9.54 μg/mL, 9.23 μg/mL for SNF-NLPs and 16.85 μg/mL for free SNF. T7-SNF-NLPs was highly efficient in suppressing the tumor growth in xenograft tumor model. The proportion of Ki67 in T7-SNF-NLPs group was significantly lower than that of either free drug or nonmodified NLPs.