LncRNA AGAP2-AS1 accelerates cell proliferation, migration, invasion and EMT process in colorectal cancer via regulating miR-4668-3p/SRSF1 axis

Colorectal Cancer
08/07/2020

Li H, et al. J Gene Med 2020.

ABSTRACT

BACKGROUND: Colorectal cancer (CRC) is a frequently occurring tumor. Although a number of long noncoding RNAs (lncRNAs) have been researched in CRC, the expression, function and mechanism of AGAP2-AS1 remains poorly investigated in CRC.

METHODS: Gene expression was analyzed by RT-qPCR and western blot analyses. CCK8, colony formation and transwell assays were utilized to explore the functional role of AGAP2-AS1 in CRC. Luciferase reporter, RNA pull down and RIP assays were implemented to verify relationships between RNA molecules.

RESULTS: In current work, AGAP2-AS1 was unveiled as highly expressed in CRC cell lines compared with normal cells. AGAP2-AS1 knockdown suppressed cell proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT) process. Interestingly, AGAP2-AS1 sponges miR-4668-3p to release SRSF1 in CRC. Furthermore, in the rescue functional assay, miR-4668-3p down-regulation exacerbated the malignant behaviors of AGAP2-AS1-depleted CRC cells, while such effects were further offset by SRSF1 knockdown.

CONCLUSIONS: AGAP2-AS1 facilitates cell proliferation, motility and EMT in CRC via targeting miR-4668-3p/SRSF1 axis. AGAP2-AS1 or SRSF1 may become underlying therapeutic targets for CRC patients in the future.