The Role of Cavin3 in the Progression of Lung Cancer and Its Mechanism

Lung Cancer
30/04/2020

Sun G and Ni K. Biomed Res Int 2020.

ABSTRACT

OBJECTIVE: The purpose of this study was to describe the role of Cavin3 in the progression of lung cancer and its underlying mechanism.

METHODS: Totally, 200 cases of lung cancer tissues and corresponding paracancer tissues were collected. Cavin3 expression in samples was determined by qRT-PCR, and the correlation with lung cancer stages as well as prognosis was statistically analyzed combined with matched clinical information. To investigate the mechanism of Cavin3 in lung cancer progression, firstly, Cavin3 was detected in lung cancer cell lines A549, PC9, and H520. Then, cells with stable Cavin3 overexpression and Cavin3 knockout were established to determine the effect of Cavin3 overexpression on the mammalian target of rapamycin (mTOR) signaling pathway. Subsequently, cells were harvested for cell proliferation, migration, and invasion assays in vitro, as well as nude mouse transplantation tumor experiment in vivo.

RESULTS: Cavin3 was seen to be highly expressed in cancer tissues. Statistical analysis with matched clinical data showed that Cavin3 as a prognostic indicator of lung cancer had important clinical value. In addition, it could be found that high expression of Cavin3 was able to promote cell proliferation, migration, and invasion and also potentiate tumor formation in vivo.

CONCLUSION: Cavin3 was highly expressed in lung cancer, and it was capable to promote cell proliferation, invasion, and migration.