Mao R, et al. Cancer Sci 2020.
PD-L1 expressed on the cancer cells can cause immune escape of NSCLC. Elucidation of the regulatory mechanisms of the PD-L1 expression is a prerequisite for establishment of new tumor immunotherapy strategies. Deubiquitinase UCHL1 is a regulator of cellular signaling transduction and expressed aberrantly in NSCLC. But it is not known whether UCHL1 regulates the expression of PD-L1 in NSCLC cells. In the present study, we found that UCHL1 promotes the expression of PD-L1 in NSCLC cell lines.
Besides, UCHL1 expressed in NSCLC cells inhibited activation of Jurkat cells through upregulation of PD-L1 expression in vitro experiments. Moreover, the mechanism for UCHL1 to upregulate PD-L1 expression is that UCHL1 facilitated activation of AKT-P65 signaling pathway. In conclusion, these results demonstrated that UCHL1 promoted PD-L1 expression in NSCLC cells. This finding implied that inhibition of UCHL1 might suppress immune escape of NSCLC via downregulation of PD-L1 expression in NSCLC cells.