Mannose shows antitumour properties against lung cancer via inhibiting proliferation, promoting cisplatin‑mediated apoptosis and reducing metastasis

Lung Cancer
23/07/2020

Mol Med Rep. 2020 Jul 23. doi: 10.3892/mmr.2020.11354. Online ahead of print.

ABSTRACT

It has been reported that mannose exerts antitumour effects against certain types of cancer. The present study was designed to evaluate whether mannose exerted potential anticancer effects on A549 and H1299 non‑small cell lung cancer (NSCLC) cells in vitro, which has not been reported previously. A Cell Counting Kit‑8 cell viability assay was used to assess the antiproliferative effects of mannose on NSCLC cells. Flow cytometry‑based methods were used to evaluate the effects of mannose on the


cell cycle distribution and cisplatin‑mediated apoptosis of NSCLC cells. Transwell migration and invasion assays were conducted to examine whether mannose could inhibit the invasive abilities of NSCLC cells. The effects of mannose on the PI3K/AKT and ERK signalling pathways were explored through western blot analysis assessing the expression of phosphorylated (p)‑AKT and p‑ERK1/2. It was found that mannose showed potential anticancer effects against NSCLC cells in vitro by inhibiting proliferation, inducing G0/G1 cell cycle arrest, promoting cisplatin‑induced apoptosis and decreasing the invasive abilities. These data indicate the potential anticancer properties of mannose and suggest the application of mannose‑based therapies to treat NSCLC.