Generation of pralatrexate resistant T-cell lymphoma lines reveals two patterns of acquired drug resistance that is overcome with epigenetic modifiers

Lymphoma
03/07/2020

Scotto L, et al. Genes Chromosomes Cancer 2020.

ABSTRACT

While pralatrexate has been successfully developed for the treatment of T-cell lymphoma, the mechanistic basis for its T-cell selectivity and acquired resistance remains elusive. In an effort to potentially identify synergistic combinations that might circumnavigate or delay acquired pralatrexate resistance, we generated resistant cells lines over a broad concentration range. Pralatrexate-resistant cell lines H9-12 and H9-200 were developed, each exhibiting an IC50 of 35 and over 1000 nM,


respectively. These lines were established in vitro from parental H9 cells. Expression analysis of the proteins known to be important determinants of antifolate pharmacology revealed increase expression of DHFR due to gene amplification, and RFC1 down-regulation, as the putative mechanisms of resistance in H9-12 and H9-200 cells. Cross resistance was only seen with methotrexate but not with romidepsin, azacitidine, decitabine, gemcitabine, doxorubicin or bortezomib. Resistance to pralatrexate was reversed by pre-treatment with hypomethylating agents in a concentration-dependent fashion. Comparison of gene expression profiles of parental and resistant cell lines confirmed markedly different patterns of gene expression, and identified the dual specificity phosphatase four (DUSP4) as one of the molecular target of pralatrexate activity. Reduced STAT5 phosphorylation following exposure to pralatrexate was observed in the H9 but not in the H9-12 and H9-200 cells. These data suggest that combination with hypomethylating agents could be potent, and that DUSP4 and STAT5 could represent putative biomarkers of pralatrexate activity. This article is protected by copyright. All rights reserved.